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n a string v11 5 online tool  (Bio-Rad)


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    Bio-Rad n a string v11 5 online tool
    N A String V11 5 Online Tool, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 4569 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+a+string+v11+5+online+tool/Bio-Plex+Manager+Software/pm36924501-524-245-261
    Average 99 stars, based on 4569 article reviews
    n a string v11 5 online tool - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: Neonatal SHIV infection in rhesus macaques elicited heterologous HIV-1-neutralizing antibodies.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human Bcl-6 BD Cat# 561522; RRID:AB_10717126 PE-CF594 anti-human CD38 Caprico Biotechnologies RRID: AB_1008204 PE-Cy5 anti-human CD69 Biolegend Cat# 310908; RRID:AB_314843 PE-Cy7 anti-human CD21 BD Cat# 561374; RRID:AB_10681717 Alexa Fluor 700 anti-human Ki67 BD Cat# 561277; RRID:AB_10611571 APC-H7 anti-human IgG BD Cat# 561297; RRID:AB_10611877 BV605 anti-human IgM BD Cat# 562977; RRID:AB_2737928 BV650 anti-human CD8 Biolegend Cat# 301042; RRID:AB_2563505 BV650 anti-human CD16 BD Cat# 563692; RRID:AB_2869511 BV650 anti-human CD3 BD Cat# 563916; RRID:AB_2738486 BV650 anti-human CD14 BD Cat# 563419; RRID:AB_2744286 BV711 anti-human CD27 Biolegend Cat# 302834; RRID:AB_2563809 BV785 anti-human CD20 Biolegend Cat# 302356; RRID:AB_2566316 BV421 anti-human PD-1 BioLegend Cat# 329920; RRID:AB_10960742 BV570 anti-human CD8a BioLegend Cat# 301038; RRID:AB_2563213 BV605 anti-human CCR7 BD Cat# 563711; RRID:AB_2738385 BV650 anti-human CD25 BioLegend Cat# 302634; RRID:AB_2563807 BV786 anti-human CD45RA BD Cat# 741010; RRID:AB_2740633 Alexa Fluor 700 anti-human CD4 BioLegend Cat# 317440; RRID:AB_2562912 APC-Cy7 anti-human CD3 BD Cat# 557757; RRID:AB_396863 BB515 anti-human ICOS BD Cat# 565880; RRID:AB_2744480 BB700 anti-human CCR6 BD Cat# 566477; RRID:AB_2744303 PE anti-human CD134 (OX40) BD Cat# 340420; RRID:AB_400027 PE-CF594 anti-human CD183 BD Cat# 562451; RRID:AB_11153118 Biotinylated anti-human CD185 (CXCR5) ThermoFisher Cat# 13-9185-82; RRID:AB_2572807 Alexa Fluor 647 anti-human CD137 BioLegend Cat# 309824; RRID:AB_2566258 PG9 Walker et al.54 PG9 DH270 Bonsignori et al.19 DH270 Streptavidin-PE-Cy7 BioLegend Cat#405206 Goat anti-human IgG-HRP Jackson ImmunoResearch Laboratories Cat#109-035-098 Mouse anti-monkey IgG-HRP Southern Biotech Cat#4700-05 Streptavidin-HRP Thermo Scientific Ref#21130 Mouse-Human chimeric mAb D001 Sino Biological Inc Cat#40150-D001 Bacterial and virus strains Simian-HIV bearing TF Envs Li et al.15 N/A SHIV CH848.10.17.E169K This paper SHIV CH848.10.17.E169K SHIV CH848 10.17 N133DN138T.E169K This paper SHIV CH848 10.17 N133DN138T.E169K 25710 deCamp et al.35 EF117271 X1632 deCamp et al.35 FJ817370 T250 Gorman et al.36 T-250 CAP256 Doria-Rose et al.23 CAP256 Q23 Gorman et al.36 Q23.17 (Continued on next page) Cell Reports 42, 112255, March 28, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER ZM233 Gorman et al.36 ZM233.6 MuLV Sarzotti-Kelsoe et al.55 MLV Chemicals, peptides, and recombinant proteins CH848 10.17 DT.E169K gp120 This paper CH848 10.17 N133DN138T.E169K gp120 CH848 10.17 DS.SOSIP_DT.E169K This paper CH848 10.17 DS.SOSIP_N133DN138T.E169K gp70_B.CaseA2 V1V2/ 169K Liao et al.56 gp70 B.CaseA2 V1-V2/V169K AE.244_V1V2 Tags Liao et al.56 AE.A244 V1-V2 tags M.CON-S V3 peptide Liao et al.57 Group M Consensus MN gp41 ImmunoDx rgp41 HIV-1; SKU - 1091 VRC_C gp70 V1V2 Liao et al.56 97ZA012-V1V2 CAP206TF_V1V2 Tags Barton Haynes, Duke University CAP206TF_V1V2 Tags gp70_C.1086C V1V2 Liao et al.22 gp70 C.1086 V1-V2 Critical commercial assays Virus/Pathogen DSP midi kit Qiagen Cat#937055 ExpiFectamine 293 transfection reagents Life Technologies, Gibco Cat#A14524 LIVE/DEAD Fixable Aqua Dead cell kit ThermoFisher Cat#L34957 FoxP3 Transcription Factor Fixation/ Permeabilization reagents ThermoFisher Cat#00-5523-00 BIOTIN-X-NHS Cayman Chemicals Cat#13316 Chromium Next GEM Automated Single Cell 50 Reagent Kits v2 10X Genomics PN-1000290 Novaseq S4 Reagent kit Illumina PN-20028313 Chromium Next GEM Chip K Automated Single Cell Kit 10X Genomics PN-1000289 Automated Library Construction Kit 10X Genomics PN-1000428 13-plex NHP ProcartaPlex panel ThermoFischer Cat#: PPX-13 Assay ID: MXH6CE4 Deposited data Single cell transcriptome data NCBI SRA PRJNA931919 CH848_1017.DT.E169K gp140 sequence GenBank OQ407502 Experimental models: cell lines HEK 293T ATCC Cat #CRL3216 Software and algorithms Cell Ranger Single Cell Software Suite (version 4.0.0) 10X Genomics N/A Seurat R package (v4.1.0) Hao et al.58 N/A SingleR (v1.6.1) Aran et al.59 N/A celldex (v1.4.0) R packages Aran et al.59 N/A Monaco Immune Data Monaco et al.60 N/A STRING v11.5 online tool https://string-db.org/ N/A FlowJo version 10.8 FlowJo LLC N/A Bio-Plex Manager v6.2 Bio-Rad N/A Geneious Prime software Biomatters N/A ..

    Virus:

    Article Title: Neonatal SHIV infection in rhesus macaques elicited heterologous HIV-1-neutralizing antibodies.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human Bcl-6 BD Cat# 561522; RRID:AB_10717126 PE-CF594 anti-human CD38 Caprico Biotechnologies RRID: AB_1008204 PE-Cy5 anti-human CD69 Biolegend Cat# 310908; RRID:AB_314843 PE-Cy7 anti-human CD21 BD Cat# 561374; RRID:AB_10681717 Alexa Fluor 700 anti-human Ki67 BD Cat# 561277; RRID:AB_10611571 APC-H7 anti-human IgG BD Cat# 561297; RRID:AB_10611877 BV605 anti-human IgM BD Cat# 562977; RRID:AB_2737928 BV650 anti-human CD8 Biolegend Cat# 301042; RRID:AB_2563505 BV650 anti-human CD16 BD Cat# 563692; RRID:AB_2869511 BV650 anti-human CD3 BD Cat# 563916; RRID:AB_2738486 BV650 anti-human CD14 BD Cat# 563419; RRID:AB_2744286 BV711 anti-human CD27 Biolegend Cat# 302834; RRID:AB_2563809 BV785 anti-human CD20 Biolegend Cat# 302356; RRID:AB_2566316 BV421 anti-human PD-1 BioLegend Cat# 329920; RRID:AB_10960742 BV570 anti-human CD8a BioLegend Cat# 301038; RRID:AB_2563213 BV605 anti-human CCR7 BD Cat# 563711; RRID:AB_2738385 BV650 anti-human CD25 BioLegend Cat# 302634; RRID:AB_2563807 BV786 anti-human CD45RA BD Cat# 741010; RRID:AB_2740633 Alexa Fluor 700 anti-human CD4 BioLegend Cat# 317440; RRID:AB_2562912 APC-Cy7 anti-human CD3 BD Cat# 557757; RRID:AB_396863 BB515 anti-human ICOS BD Cat# 565880; RRID:AB_2744480 BB700 anti-human CCR6 BD Cat# 566477; RRID:AB_2744303 PE anti-human CD134 (OX40) BD Cat# 340420; RRID:AB_400027 PE-CF594 anti-human CD183 BD Cat# 562451; RRID:AB_11153118 Biotinylated anti-human CD185 (CXCR5) ThermoFisher Cat# 13-9185-82; RRID:AB_2572807 Alexa Fluor 647 anti-human CD137 BioLegend Cat# 309824; RRID:AB_2566258 PG9 Walker et al.54 PG9 DH270 Bonsignori et al.19 DH270 Streptavidin-PE-Cy7 BioLegend Cat#405206 Goat anti-human IgG-HRP Jackson ImmunoResearch Laboratories Cat#109-035-098 Mouse anti-monkey IgG-HRP Southern Biotech Cat#4700-05 Streptavidin-HRP Thermo Scientific Ref#21130 Mouse-Human chimeric mAb D001 Sino Biological Inc Cat#40150-D001 Bacterial and virus strains Simian-HIV bearing TF Envs Li et al.15 N/A SHIV CH848.10.17.E169K This paper SHIV CH848.10.17.E169K SHIV CH848 10.17 N133DN138T.E169K This paper SHIV CH848 10.17 N133DN138T.E169K 25710 deCamp et al.35 EF117271 X1632 deCamp et al.35 FJ817370 T250 Gorman et al.36 T-250 CAP256 Doria-Rose et al.23 CAP256 Q23 Gorman et al.36 Q23.17 (Continued on next page) Cell Reports 42, 112255, March 28, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER ZM233 Gorman et al.36 ZM233.6 MuLV Sarzotti-Kelsoe et al.55 MLV Chemicals, peptides, and recombinant proteins CH848 10.17 DT.E169K gp120 This paper CH848 10.17 N133DN138T.E169K gp120 CH848 10.17 DS.SOSIP_DT.E169K This paper CH848 10.17 DS.SOSIP_N133DN138T.E169K gp70_B.CaseA2 V1V2/ 169K Liao et al.56 gp70 B.CaseA2 V1-V2/V169K AE.244_V1V2 Tags Liao et al.56 AE.A244 V1-V2 tags M.CON-S V3 peptide Liao et al.57 Group M Consensus MN gp41 ImmunoDx rgp41 HIV-1; SKU - 1091 VRC_C gp70 V1V2 Liao et al.56 97ZA012-V1V2 CAP206TF_V1V2 Tags Barton Haynes, Duke University CAP206TF_V1V2 Tags gp70_C.1086C V1V2 Liao et al.22 gp70 C.1086 V1-V2 Critical commercial assays Virus/Pathogen DSP midi kit Qiagen Cat#937055 ExpiFectamine 293 transfection reagents Life Technologies, Gibco Cat#A14524 LIVE/DEAD Fixable Aqua Dead cell kit ThermoFisher Cat#L34957 FoxP3 Transcription Factor Fixation/ Permeabilization reagents ThermoFisher Cat#00-5523-00 BIOTIN-X-NHS Cayman Chemicals Cat#13316 Chromium Next GEM Automated Single Cell 50 Reagent Kits v2 10X Genomics PN-1000290 Novaseq S4 Reagent kit Illumina PN-20028313 Chromium Next GEM Chip K Automated Single Cell Kit 10X Genomics PN-1000289 Automated Library Construction Kit 10X Genomics PN-1000428 13-plex NHP ProcartaPlex panel ThermoFischer Cat#: PPX-13 Assay ID: MXH6CE4 Deposited data Single cell transcriptome data NCBI SRA PRJNA931919 CH848_1017.DT.E169K gp140 sequence GenBank OQ407502 Experimental models: cell lines HEK 293T ATCC Cat #CRL3216 Software and algorithms Cell Ranger Single Cell Software Suite (version 4.0.0) 10X Genomics N/A Seurat R package (v4.1.0) Hao et al.58 N/A SingleR (v1.6.1) Aran et al.59 N/A celldex (v1.4.0) R packages Aran et al.59 N/A Monaco Immune Data Monaco et al.60 N/A STRING v11.5 online tool https://string-db.org/ N/A FlowJo version 10.8 FlowJo LLC N/A Bio-Plex Manager v6.2 Bio-Rad N/A Geneious Prime software Biomatters N/A ..

    Transfection:

    Article Title: Neonatal SHIV infection in rhesus macaques elicited heterologous HIV-1-neutralizing antibodies.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human Bcl-6 BD Cat# 561522; RRID:AB_10717126 PE-CF594 anti-human CD38 Caprico Biotechnologies RRID: AB_1008204 PE-Cy5 anti-human CD69 Biolegend Cat# 310908; RRID:AB_314843 PE-Cy7 anti-human CD21 BD Cat# 561374; RRID:AB_10681717 Alexa Fluor 700 anti-human Ki67 BD Cat# 561277; RRID:AB_10611571 APC-H7 anti-human IgG BD Cat# 561297; RRID:AB_10611877 BV605 anti-human IgM BD Cat# 562977; RRID:AB_2737928 BV650 anti-human CD8 Biolegend Cat# 301042; RRID:AB_2563505 BV650 anti-human CD16 BD Cat# 563692; RRID:AB_2869511 BV650 anti-human CD3 BD Cat# 563916; RRID:AB_2738486 BV650 anti-human CD14 BD Cat# 563419; RRID:AB_2744286 BV711 anti-human CD27 Biolegend Cat# 302834; RRID:AB_2563809 BV785 anti-human CD20 Biolegend Cat# 302356; RRID:AB_2566316 BV421 anti-human PD-1 BioLegend Cat# 329920; RRID:AB_10960742 BV570 anti-human CD8a BioLegend Cat# 301038; RRID:AB_2563213 BV605 anti-human CCR7 BD Cat# 563711; RRID:AB_2738385 BV650 anti-human CD25 BioLegend Cat# 302634; RRID:AB_2563807 BV786 anti-human CD45RA BD Cat# 741010; RRID:AB_2740633 Alexa Fluor 700 anti-human CD4 BioLegend Cat# 317440; RRID:AB_2562912 APC-Cy7 anti-human CD3 BD Cat# 557757; RRID:AB_396863 BB515 anti-human ICOS BD Cat# 565880; RRID:AB_2744480 BB700 anti-human CCR6 BD Cat# 566477; RRID:AB_2744303 PE anti-human CD134 (OX40) BD Cat# 340420; RRID:AB_400027 PE-CF594 anti-human CD183 BD Cat# 562451; RRID:AB_11153118 Biotinylated anti-human CD185 (CXCR5) ThermoFisher Cat# 13-9185-82; RRID:AB_2572807 Alexa Fluor 647 anti-human CD137 BioLegend Cat# 309824; RRID:AB_2566258 PG9 Walker et al.54 PG9 DH270 Bonsignori et al.19 DH270 Streptavidin-PE-Cy7 BioLegend Cat#405206 Goat anti-human IgG-HRP Jackson ImmunoResearch Laboratories Cat#109-035-098 Mouse anti-monkey IgG-HRP Southern Biotech Cat#4700-05 Streptavidin-HRP Thermo Scientific Ref#21130 Mouse-Human chimeric mAb D001 Sino Biological Inc Cat#40150-D001 Bacterial and virus strains Simian-HIV bearing TF Envs Li et al.15 N/A SHIV CH848.10.17.E169K This paper SHIV CH848.10.17.E169K SHIV CH848 10.17 N133DN138T.E169K This paper SHIV CH848 10.17 N133DN138T.E169K 25710 deCamp et al.35 EF117271 X1632 deCamp et al.35 FJ817370 T250 Gorman et al.36 T-250 CAP256 Doria-Rose et al.23 CAP256 Q23 Gorman et al.36 Q23.17 (Continued on next page) Cell Reports 42, 112255, March 28, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER ZM233 Gorman et al.36 ZM233.6 MuLV Sarzotti-Kelsoe et al.55 MLV Chemicals, peptides, and recombinant proteins CH848 10.17 DT.E169K gp120 This paper CH848 10.17 N133DN138T.E169K gp120 CH848 10.17 DS.SOSIP_DT.E169K This paper CH848 10.17 DS.SOSIP_N133DN138T.E169K gp70_B.CaseA2 V1V2/ 169K Liao et al.56 gp70 B.CaseA2 V1-V2/V169K AE.244_V1V2 Tags Liao et al.56 AE.A244 V1-V2 tags M.CON-S V3 peptide Liao et al.57 Group M Consensus MN gp41 ImmunoDx rgp41 HIV-1; SKU - 1091 VRC_C gp70 V1V2 Liao et al.56 97ZA012-V1V2 CAP206TF_V1V2 Tags Barton Haynes, Duke University CAP206TF_V1V2 Tags gp70_C.1086C V1V2 Liao et al.22 gp70 C.1086 V1-V2 Critical commercial assays Virus/Pathogen DSP midi kit Qiagen Cat#937055 ExpiFectamine 293 transfection reagents Life Technologies, Gibco Cat#A14524 LIVE/DEAD Fixable Aqua Dead cell kit ThermoFisher Cat#L34957 FoxP3 Transcription Factor Fixation/ Permeabilization reagents ThermoFisher Cat#00-5523-00 BIOTIN-X-NHS Cayman Chemicals Cat#13316 Chromium Next GEM Automated Single Cell 50 Reagent Kits v2 10X Genomics PN-1000290 Novaseq S4 Reagent kit Illumina PN-20028313 Chromium Next GEM Chip K Automated Single Cell Kit 10X Genomics PN-1000289 Automated Library Construction Kit 10X Genomics PN-1000428 13-plex NHP ProcartaPlex panel ThermoFischer Cat#: PPX-13 Assay ID: MXH6CE4 Deposited data Single cell transcriptome data NCBI SRA PRJNA931919 CH848_1017.DT.E169K gp140 sequence GenBank OQ407502 Experimental models: cell lines HEK 293T ATCC Cat #CRL3216 Software and algorithms Cell Ranger Single Cell Software Suite (version 4.0.0) 10X Genomics N/A Seurat R package (v4.1.0) Hao et al.58 N/A SingleR (v1.6.1) Aran et al.59 N/A celldex (v1.4.0) R packages Aran et al.59 N/A Monaco Immune Data Monaco et al.60 N/A STRING v11.5 online tool https://string-db.org/ N/A FlowJo version 10.8 FlowJo LLC N/A Bio-Plex Manager v6.2 Bio-Rad N/A Geneious Prime software Biomatters N/A ..

    Single Cell:

    Article Title: Neonatal SHIV infection in rhesus macaques elicited heterologous HIV-1-neutralizing antibodies.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human Bcl-6 BD Cat# 561522; RRID:AB_10717126 PE-CF594 anti-human CD38 Caprico Biotechnologies RRID: AB_1008204 PE-Cy5 anti-human CD69 Biolegend Cat# 310908; RRID:AB_314843 PE-Cy7 anti-human CD21 BD Cat# 561374; RRID:AB_10681717 Alexa Fluor 700 anti-human Ki67 BD Cat# 561277; RRID:AB_10611571 APC-H7 anti-human IgG BD Cat# 561297; RRID:AB_10611877 BV605 anti-human IgM BD Cat# 562977; RRID:AB_2737928 BV650 anti-human CD8 Biolegend Cat# 301042; RRID:AB_2563505 BV650 anti-human CD16 BD Cat# 563692; RRID:AB_2869511 BV650 anti-human CD3 BD Cat# 563916; RRID:AB_2738486 BV650 anti-human CD14 BD Cat# 563419; RRID:AB_2744286 BV711 anti-human CD27 Biolegend Cat# 302834; RRID:AB_2563809 BV785 anti-human CD20 Biolegend Cat# 302356; RRID:AB_2566316 BV421 anti-human PD-1 BioLegend Cat# 329920; RRID:AB_10960742 BV570 anti-human CD8a BioLegend Cat# 301038; RRID:AB_2563213 BV605 anti-human CCR7 BD Cat# 563711; RRID:AB_2738385 BV650 anti-human CD25 BioLegend Cat# 302634; RRID:AB_2563807 BV786 anti-human CD45RA BD Cat# 741010; RRID:AB_2740633 Alexa Fluor 700 anti-human CD4 BioLegend Cat# 317440; RRID:AB_2562912 APC-Cy7 anti-human CD3 BD Cat# 557757; RRID:AB_396863 BB515 anti-human ICOS BD Cat# 565880; RRID:AB_2744480 BB700 anti-human CCR6 BD Cat# 566477; RRID:AB_2744303 PE anti-human CD134 (OX40) BD Cat# 340420; RRID:AB_400027 PE-CF594 anti-human CD183 BD Cat# 562451; RRID:AB_11153118 Biotinylated anti-human CD185 (CXCR5) ThermoFisher Cat# 13-9185-82; RRID:AB_2572807 Alexa Fluor 647 anti-human CD137 BioLegend Cat# 309824; RRID:AB_2566258 PG9 Walker et al.54 PG9 DH270 Bonsignori et al.19 DH270 Streptavidin-PE-Cy7 BioLegend Cat#405206 Goat anti-human IgG-HRP Jackson ImmunoResearch Laboratories Cat#109-035-098 Mouse anti-monkey IgG-HRP Southern Biotech Cat#4700-05 Streptavidin-HRP Thermo Scientific Ref#21130 Mouse-Human chimeric mAb D001 Sino Biological Inc Cat#40150-D001 Bacterial and virus strains Simian-HIV bearing TF Envs Li et al.15 N/A SHIV CH848.10.17.E169K This paper SHIV CH848.10.17.E169K SHIV CH848 10.17 N133DN138T.E169K This paper SHIV CH848 10.17 N133DN138T.E169K 25710 deCamp et al.35 EF117271 X1632 deCamp et al.35 FJ817370 T250 Gorman et al.36 T-250 CAP256 Doria-Rose et al.23 CAP256 Q23 Gorman et al.36 Q23.17 (Continued on next page) Cell Reports 42, 112255, March 28, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER ZM233 Gorman et al.36 ZM233.6 MuLV Sarzotti-Kelsoe et al.55 MLV Chemicals, peptides, and recombinant proteins CH848 10.17 DT.E169K gp120 This paper CH848 10.17 N133DN138T.E169K gp120 CH848 10.17 DS.SOSIP_DT.E169K This paper CH848 10.17 DS.SOSIP_N133DN138T.E169K gp70_B.CaseA2 V1V2/ 169K Liao et al.56 gp70 B.CaseA2 V1-V2/V169K AE.244_V1V2 Tags Liao et al.56 AE.A244 V1-V2 tags M.CON-S V3 peptide Liao et al.57 Group M Consensus MN gp41 ImmunoDx rgp41 HIV-1; SKU - 1091 VRC_C gp70 V1V2 Liao et al.56 97ZA012-V1V2 CAP206TF_V1V2 Tags Barton Haynes, Duke University CAP206TF_V1V2 Tags gp70_C.1086C V1V2 Liao et al.22 gp70 C.1086 V1-V2 Critical commercial assays Virus/Pathogen DSP midi kit Qiagen Cat#937055 ExpiFectamine 293 transfection reagents Life Technologies, Gibco Cat#A14524 LIVE/DEAD Fixable Aqua Dead cell kit ThermoFisher Cat#L34957 FoxP3 Transcription Factor Fixation/ Permeabilization reagents ThermoFisher Cat#00-5523-00 BIOTIN-X-NHS Cayman Chemicals Cat#13316 Chromium Next GEM Automated Single Cell 50 Reagent Kits v2 10X Genomics PN-1000290 Novaseq S4 Reagent kit Illumina PN-20028313 Chromium Next GEM Chip K Automated Single Cell Kit 10X Genomics PN-1000289 Automated Library Construction Kit 10X Genomics PN-1000428 13-plex NHP ProcartaPlex panel ThermoFischer Cat#: PPX-13 Assay ID: MXH6CE4 Deposited data Single cell transcriptome data NCBI SRA PRJNA931919 CH848_1017.DT.E169K gp140 sequence GenBank OQ407502 Experimental models: cell lines HEK 293T ATCC Cat #CRL3216 Software and algorithms Cell Ranger Single Cell Software Suite (version 4.0.0) 10X Genomics N/A Seurat R package (v4.1.0) Hao et al.58 N/A SingleR (v1.6.1) Aran et al.59 N/A celldex (v1.4.0) R packages Aran et al.59 N/A Monaco Immune Data Monaco et al.60 N/A STRING v11.5 online tool https://string-db.org/ N/A FlowJo version 10.8 FlowJo LLC N/A Bio-Plex Manager v6.2 Bio-Rad N/A Geneious Prime software Biomatters N/A ..

    Sequencing:

    Article Title: Neonatal SHIV infection in rhesus macaques elicited heterologous HIV-1-neutralizing antibodies.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human Bcl-6 BD Cat# 561522; RRID:AB_10717126 PE-CF594 anti-human CD38 Caprico Biotechnologies RRID: AB_1008204 PE-Cy5 anti-human CD69 Biolegend Cat# 310908; RRID:AB_314843 PE-Cy7 anti-human CD21 BD Cat# 561374; RRID:AB_10681717 Alexa Fluor 700 anti-human Ki67 BD Cat# 561277; RRID:AB_10611571 APC-H7 anti-human IgG BD Cat# 561297; RRID:AB_10611877 BV605 anti-human IgM BD Cat# 562977; RRID:AB_2737928 BV650 anti-human CD8 Biolegend Cat# 301042; RRID:AB_2563505 BV650 anti-human CD16 BD Cat# 563692; RRID:AB_2869511 BV650 anti-human CD3 BD Cat# 563916; RRID:AB_2738486 BV650 anti-human CD14 BD Cat# 563419; RRID:AB_2744286 BV711 anti-human CD27 Biolegend Cat# 302834; RRID:AB_2563809 BV785 anti-human CD20 Biolegend Cat# 302356; RRID:AB_2566316 BV421 anti-human PD-1 BioLegend Cat# 329920; RRID:AB_10960742 BV570 anti-human CD8a BioLegend Cat# 301038; RRID:AB_2563213 BV605 anti-human CCR7 BD Cat# 563711; RRID:AB_2738385 BV650 anti-human CD25 BioLegend Cat# 302634; RRID:AB_2563807 BV786 anti-human CD45RA BD Cat# 741010; RRID:AB_2740633 Alexa Fluor 700 anti-human CD4 BioLegend Cat# 317440; RRID:AB_2562912 APC-Cy7 anti-human CD3 BD Cat# 557757; RRID:AB_396863 BB515 anti-human ICOS BD Cat# 565880; RRID:AB_2744480 BB700 anti-human CCR6 BD Cat# 566477; RRID:AB_2744303 PE anti-human CD134 (OX40) BD Cat# 340420; RRID:AB_400027 PE-CF594 anti-human CD183 BD Cat# 562451; RRID:AB_11153118 Biotinylated anti-human CD185 (CXCR5) ThermoFisher Cat# 13-9185-82; RRID:AB_2572807 Alexa Fluor 647 anti-human CD137 BioLegend Cat# 309824; RRID:AB_2566258 PG9 Walker et al.54 PG9 DH270 Bonsignori et al.19 DH270 Streptavidin-PE-Cy7 BioLegend Cat#405206 Goat anti-human IgG-HRP Jackson ImmunoResearch Laboratories Cat#109-035-098 Mouse anti-monkey IgG-HRP Southern Biotech Cat#4700-05 Streptavidin-HRP Thermo Scientific Ref#21130 Mouse-Human chimeric mAb D001 Sino Biological Inc Cat#40150-D001 Bacterial and virus strains Simian-HIV bearing TF Envs Li et al.15 N/A SHIV CH848.10.17.E169K This paper SHIV CH848.10.17.E169K SHIV CH848 10.17 N133DN138T.E169K This paper SHIV CH848 10.17 N133DN138T.E169K 25710 deCamp et al.35 EF117271 X1632 deCamp et al.35 FJ817370 T250 Gorman et al.36 T-250 CAP256 Doria-Rose et al.23 CAP256 Q23 Gorman et al.36 Q23.17 (Continued on next page) Cell Reports 42, 112255, March 28, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER ZM233 Gorman et al.36 ZM233.6 MuLV Sarzotti-Kelsoe et al.55 MLV Chemicals, peptides, and recombinant proteins CH848 10.17 DT.E169K gp120 This paper CH848 10.17 N133DN138T.E169K gp120 CH848 10.17 DS.SOSIP_DT.E169K This paper CH848 10.17 DS.SOSIP_N133DN138T.E169K gp70_B.CaseA2 V1V2/ 169K Liao et al.56 gp70 B.CaseA2 V1-V2/V169K AE.244_V1V2 Tags Liao et al.56 AE.A244 V1-V2 tags M.CON-S V3 peptide Liao et al.57 Group M Consensus MN gp41 ImmunoDx rgp41 HIV-1; SKU - 1091 VRC_C gp70 V1V2 Liao et al.56 97ZA012-V1V2 CAP206TF_V1V2 Tags Barton Haynes, Duke University CAP206TF_V1V2 Tags gp70_C.1086C V1V2 Liao et al.22 gp70 C.1086 V1-V2 Critical commercial assays Virus/Pathogen DSP midi kit Qiagen Cat#937055 ExpiFectamine 293 transfection reagents Life Technologies, Gibco Cat#A14524 LIVE/DEAD Fixable Aqua Dead cell kit ThermoFisher Cat#L34957 FoxP3 Transcription Factor Fixation/ Permeabilization reagents ThermoFisher Cat#00-5523-00 BIOTIN-X-NHS Cayman Chemicals Cat#13316 Chromium Next GEM Automated Single Cell 50 Reagent Kits v2 10X Genomics PN-1000290 Novaseq S4 Reagent kit Illumina PN-20028313 Chromium Next GEM Chip K Automated Single Cell Kit 10X Genomics PN-1000289 Automated Library Construction Kit 10X Genomics PN-1000428 13-plex NHP ProcartaPlex panel ThermoFischer Cat#: PPX-13 Assay ID: MXH6CE4 Deposited data Single cell transcriptome data NCBI SRA PRJNA931919 CH848_1017.DT.E169K gp140 sequence GenBank OQ407502 Experimental models: cell lines HEK 293T ATCC Cat #CRL3216 Software and algorithms Cell Ranger Single Cell Software Suite (version 4.0.0) 10X Genomics N/A Seurat R package (v4.1.0) Hao et al.58 N/A SingleR (v1.6.1) Aran et al.59 N/A celldex (v1.4.0) R packages Aran et al.59 N/A Monaco Immune Data Monaco et al.60 N/A STRING v11.5 online tool https://string-db.org/ N/A FlowJo version 10.8 FlowJo LLC N/A Bio-Plex Manager v6.2 Bio-Rad N/A Geneious Prime software Biomatters N/A ..

    Software:

    Article Title: Neonatal SHIV infection in rhesus macaques elicited heterologous HIV-1-neutralizing antibodies.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human Bcl-6 BD Cat# 561522; RRID:AB_10717126 PE-CF594 anti-human CD38 Caprico Biotechnologies RRID: AB_1008204 PE-Cy5 anti-human CD69 Biolegend Cat# 310908; RRID:AB_314843 PE-Cy7 anti-human CD21 BD Cat# 561374; RRID:AB_10681717 Alexa Fluor 700 anti-human Ki67 BD Cat# 561277; RRID:AB_10611571 APC-H7 anti-human IgG BD Cat# 561297; RRID:AB_10611877 BV605 anti-human IgM BD Cat# 562977; RRID:AB_2737928 BV650 anti-human CD8 Biolegend Cat# 301042; RRID:AB_2563505 BV650 anti-human CD16 BD Cat# 563692; RRID:AB_2869511 BV650 anti-human CD3 BD Cat# 563916; RRID:AB_2738486 BV650 anti-human CD14 BD Cat# 563419; RRID:AB_2744286 BV711 anti-human CD27 Biolegend Cat# 302834; RRID:AB_2563809 BV785 anti-human CD20 Biolegend Cat# 302356; RRID:AB_2566316 BV421 anti-human PD-1 BioLegend Cat# 329920; RRID:AB_10960742 BV570 anti-human CD8a BioLegend Cat# 301038; RRID:AB_2563213 BV605 anti-human CCR7 BD Cat# 563711; RRID:AB_2738385 BV650 anti-human CD25 BioLegend Cat# 302634; RRID:AB_2563807 BV786 anti-human CD45RA BD Cat# 741010; RRID:AB_2740633 Alexa Fluor 700 anti-human CD4 BioLegend Cat# 317440; RRID:AB_2562912 APC-Cy7 anti-human CD3 BD Cat# 557757; RRID:AB_396863 BB515 anti-human ICOS BD Cat# 565880; RRID:AB_2744480 BB700 anti-human CCR6 BD Cat# 566477; RRID:AB_2744303 PE anti-human CD134 (OX40) BD Cat# 340420; RRID:AB_400027 PE-CF594 anti-human CD183 BD Cat# 562451; RRID:AB_11153118 Biotinylated anti-human CD185 (CXCR5) ThermoFisher Cat# 13-9185-82; RRID:AB_2572807 Alexa Fluor 647 anti-human CD137 BioLegend Cat# 309824; RRID:AB_2566258 PG9 Walker et al.54 PG9 DH270 Bonsignori et al.19 DH270 Streptavidin-PE-Cy7 BioLegend Cat#405206 Goat anti-human IgG-HRP Jackson ImmunoResearch Laboratories Cat#109-035-098 Mouse anti-monkey IgG-HRP Southern Biotech Cat#4700-05 Streptavidin-HRP Thermo Scientific Ref#21130 Mouse-Human chimeric mAb D001 Sino Biological Inc Cat#40150-D001 Bacterial and virus strains Simian-HIV bearing TF Envs Li et al.15 N/A SHIV CH848.10.17.E169K This paper SHIV CH848.10.17.E169K SHIV CH848 10.17 N133DN138T.E169K This paper SHIV CH848 10.17 N133DN138T.E169K 25710 deCamp et al.35 EF117271 X1632 deCamp et al.35 FJ817370 T250 Gorman et al.36 T-250 CAP256 Doria-Rose et al.23 CAP256 Q23 Gorman et al.36 Q23.17 (Continued on next page) Cell Reports 42, 112255, March 28, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER ZM233 Gorman et al.36 ZM233.6 MuLV Sarzotti-Kelsoe et al.55 MLV Chemicals, peptides, and recombinant proteins CH848 10.17 DT.E169K gp120 This paper CH848 10.17 N133DN138T.E169K gp120 CH848 10.17 DS.SOSIP_DT.E169K This paper CH848 10.17 DS.SOSIP_N133DN138T.E169K gp70_B.CaseA2 V1V2/ 169K Liao et al.56 gp70 B.CaseA2 V1-V2/V169K AE.244_V1V2 Tags Liao et al.56 AE.A244 V1-V2 tags M.CON-S V3 peptide Liao et al.57 Group M Consensus MN gp41 ImmunoDx rgp41 HIV-1; SKU - 1091 VRC_C gp70 V1V2 Liao et al.56 97ZA012-V1V2 CAP206TF_V1V2 Tags Barton Haynes, Duke University CAP206TF_V1V2 Tags gp70_C.1086C V1V2 Liao et al.22 gp70 C.1086 V1-V2 Critical commercial assays Virus/Pathogen DSP midi kit Qiagen Cat#937055 ExpiFectamine 293 transfection reagents Life Technologies, Gibco Cat#A14524 LIVE/DEAD Fixable Aqua Dead cell kit ThermoFisher Cat#L34957 FoxP3 Transcription Factor Fixation/ Permeabilization reagents ThermoFisher Cat#00-5523-00 BIOTIN-X-NHS Cayman Chemicals Cat#13316 Chromium Next GEM Automated Single Cell 50 Reagent Kits v2 10X Genomics PN-1000290 Novaseq S4 Reagent kit Illumina PN-20028313 Chromium Next GEM Chip K Automated Single Cell Kit 10X Genomics PN-1000289 Automated Library Construction Kit 10X Genomics PN-1000428 13-plex NHP ProcartaPlex panel ThermoFischer Cat#: PPX-13 Assay ID: MXH6CE4 Deposited data Single cell transcriptome data NCBI SRA PRJNA931919 CH848_1017.DT.E169K gp140 sequence GenBank OQ407502 Experimental models: cell lines HEK 293T ATCC Cat #CRL3216 Software and algorithms Cell Ranger Single Cell Software Suite (version 4.0.0) 10X Genomics N/A Seurat R package (v4.1.0) Hao et al.58 N/A SingleR (v1.6.1) Aran et al.59 N/A celldex (v1.4.0) R packages Aran et al.59 N/A Monaco Immune Data Monaco et al.60 N/A STRING v11.5 online tool https://string-db.org/ N/A FlowJo version 10.8 FlowJo LLC N/A Bio-Plex Manager v6.2 Bio-Rad N/A Geneious Prime software Biomatters N/A ..



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